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Healthy untreated cells were considered as negative control for the assay, whereas cells treated with 1% Triton X-100 were considered as the positive control for maximum LDH release

At the national level, the Indian government implemented the Cigarettes and Other Tobacco Products Act (COTPA) in 2003 and followed this with the ratification of the World Health Organizations Framework Convention on Tobacco Control (WHO FCTC) in 2004 17

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Quitting smoking among adults United States, 20002015

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Its buzz (high/lightheadedness) comes a little slower but hits very hard and lasts long, especially in the first 5-10 minutes